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| García,Radamés L.; Feraud,Dania; Lugo,Sonia; Machado,Héctor; Abeledo,María A.. |
| Con el objetivo de comparar un ELISA indirecto elaborado a partir de Leptospira biflexa serogrupo Semaranga serovar Patoc, con la técnica de aglutinación microscópica (de su sigla en inglés MAT) en el diagnóstico serológico de la leptospirosis canina, se utilizaron 77 muestras de suero sanguíneo de caninos con sospecha de leptospirosis (Grupo 1) y 84 muestras de animales aparentemente sanos (Grupo 2), los cuales fueron investigados simultáneamente por el ensayo inmunoenzimático y por la técnica de aglutinación microscópica (MAT). Los valores de prevalencia obtenidos por el MAT y el ELISA, así como entre ambos grupos no mostraron diferencias significativas, lo que indica la coincidencia entre ambos métodos y la existencia de una amplia circulación de cepas... |
| Tipo: Journal article |
Palavras-chave: Leptospirosis canina; Diagnóstico serológico; ELISA; MAT. |
| Ano: 2014 |
URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S0253-570X2014000200007 |
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| Silva,Rodrigo Costa da; Machado,Gustavo Puglia; Cruvinel,Tatiane Morosini de Andrade; Cruvinel,Ciro Alexandre; Langoni,Helio. |
| Background Toxoplasmosis is a worldwide zoonosis caused by an obligate intracellular protozoan parasite,Toxoplasma gondii, that affects all warm-blooded animals, including wild animals. The increased number of cases of parasitic infections is mainly due to the destruction of environmental conservation areas, which is driving wild animals out of their habitats and towards urban areas. In this study, the occurrence of T. gondii infection was investigated by the modified agglutination test (MAT) in 26 different species of run over and injured wild animals that were treated at a Brazilian university veterinary hospital, from June 2007 to August 2008.Findings Of the studied animals, six (23.1%; CI95% 11.1-42.2%) had T. gondii antibodies, with titers equal to 10... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Toxoplasma gondii; Natural infection; Antibodies; Wild animals; MAT. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992014000200603 |
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| Rodrigues, Taizi; Oliveira, Fernanda Santana; Oliveira, Vinícius; Curvelo, Vitor; Oriá, Arianne Pontes; Pinna, Melissa Hanzen. |
| Foi efetuado um inquérito sorológico da leptospirose em diferentes espécies de mamíferos silvestres, mantidos em cativeiro. Foram colhidas amostras de 88 animais, dos quais 40 primatas, 24 felídeos, 15 cervídeos, oito canídeos e um animal da Família Ursidea, totalizando 18 espécies. Os animais foram contidos quimicamente com a associação de fármacos sedativos, e posteriormente foram submetidos aos exames físicos e coleta de sangue. Após a coleta, as amostras foram resfriadas e transportadas até o laboratório para centrifugação, sendo o soro estocado. O diagnóstico sorológico para leptospirose foi realizado de acordo com a recomendação técnica da OMS, por meio da soroaglutinação microscópica (MAT) com antígenos vivos, com cada amostra sorológica testada... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Leptospirose; MAT; Animais silvestres. |
| Ano: 2013 |
URL: http://www.revistamvez-crmvsp.com.br/index.php/recmvz/article/view/21409 |
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| Fornazari,F; Camossi,LG; Silva,RC; Guazzelli,A; Ribeiro,MG; Chiacchio,SB; Langoni,H. |
| In the recent years, the wild boar (Sus scrofa) trade has increased in Brazil. The present study aimed to detect Leptospira spp. infection in 308 blood samples from wild boars bred in São Paulo state, Brazil. The microscopic agglutination test (MAT) was performed using 29 serovars. Sixty three (63; 20.45%) animals tested positive against the serovars Hardjo (29/63; 46.0%), Copenhageni (11/63; 17.4%), Pomona (8/63; 12.7%), Pyrogenes (4/63; 6.3%), Wolfii (4/63; 6.3%), Autumnalis (3/63; 4.7%), Icterohaemorraghiae (2/63; 3.1%), and Hardjo-miniswajezak (1/63; 15.8%). These results indicate that captive wild boars were infected with Leptospira spp. and may represent a source of infection to humans and other animals. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Wild boar; Sus scrofa; Leptospira spp.; MAT; Zoonosis. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992011000100012 |
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| Tomich,Renata Graça Pinto; Bomfim,Maria Rosa Quaresma; Koury,Matilde Cota; Pellegrin,Aiesca Oliveira; Pellegrin,Luiz Alberto; Ko,Albert Icksang; Barbosa-Stancioli,Edel Figueiredo. |
| This investigation was carried out in Brazilian Pantanal: region with important biodiversity. This region's climatic conditions, hydrology and geomorphology as well as the existence of great variety of wild species favor the maintenance of the Leptospira in the environment. The aim of this study was to evaluate IgG ELISA with recombinant protein LipL32 in comparison with microscopic agglutination test (MAT) and additionally contribute to the knowledge of the distribution of the one of most important worldwide zoonotic infection, assessing the seropositivity of bovine leptospirosis in beef cattle herds of Brazilian Pantanal, an important ecological preserved area, where cattle constitute not only the most important economic resource but also the major... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bovine Leptospirosis; IgG ELISA rLipL32; MAT. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000400017 |
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| Consalter,Angélica; Zanella,Eraldo Lourenso; Martins,Gabriel Mendes de Souza; Araujo,Hugo Libonati de; Lilenbaum,Walter; Souza,Guilherme Nunes de; Ferreira,Ana Maria Reis. |
| ABSTRACT: Leptospirosis is a zoonosis that is widely distributed in tropical countries. This infection is also associated with reproductive losses in livestock, which has a significant economic impact. The objective of this study is to investigate the seroprevalence and risk factors associated with leptospiral infection in commercial sheep flocks from the northwestern mesoregion of Rio Grande do Sul. Serum samples were analyzed in 319 sheep using the Microscopic Agglutination Test (MAT). At the time of sampling, an individual and herd questionnaire was carried out to investigate the risk factors. On an individual level, positive reactions were observed in 5.6% (18/319) of the studied sera, with Sejroe being the most prevalent serogroup. Animals >3 years... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Leptospira spp.; Sheep; MAT; Seroprevalence.. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782019001200451 |
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